Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #74293)


Item Catalog # Description Quantity Price (USD)
Plasmid 74293 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 10415
  • Total vector size (bp) 10694
  • Vector type
    Mammalian Expression, Bacterial Expression, CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number

Gene/Insert 1

  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • Promoter Lac promoter

Cloning Information for Gene/Insert 1

  • Cloning method Gateway Cloning
  • 5′ sequencing primer SP6 Forward: ATTTAGGTGACACTATAGA
  • 3′ sequencing primer none
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
  • Alt name
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
  • Species
  • Promoter SV40

Cloning Information for Gene/Insert 3

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See Supporting information file 1: "Instructions for one-step gRNA cloning into All-in-one CRISPR/Cas9 LacZ" from associated publication for gRNA cloning instructions using this plasmid.

Please cite associated publication when using this plasmid for cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    All_in_one_CRISPR/Cas9_LacZ was a gift from Lynne Postovit (Addgene plasmid # 74293 ; ; RRID:Addgene_74293)
  • For your References section:

    A Digital PCR-Based Method for Efficient and Highly Specific Screening of Genome Edited Cells. Findlay SD, Vincent KM, Berman JR, Postovit LM. PLoS One. 2016 Apr 18;11(4):e0153901. doi: 10.1371/journal.pone.0153901. eCollection 2016. PONE-D-16-05005 [pii] PubMed 27089539