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Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3.
Betschinger J, Nichols J, Dietmann S, Corrin PD, Paddison PJ, Smith A
Cell. 2013 Apr 11;153(2):335-47. doi: 10.1016/j.cell.2013.03.012.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
48753pPB-CAG-empty-pgk-hphPiggyBac backbone, CAG promoter, Hygromycin selection
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48754pPB-CAG-3xFLAG-empty-pgk-hphPiggyBac backbone, CAG promoter, Hygromycin selection, N-terminal 3xFLAG tag
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48755pPB-CAG-Tfe3-pgk-hphPiggyBac backbone, CAG promoter driving murine Tfe3, Hygromycin selection
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48756pPB-CAG-Tfe3::ERT2-pgk-hphPiggyBac backbone, CAG promoter driving murine Tfe3-ERT2 fusion, Hygromycin selection
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48757pPB-CAG-3xFLAG::Flcn-pgk-hphPiggyBac backbone, CAG promoter driving murine 3xFLAG-Flcn fusion, Hygromycin selection
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48758pPB-CAG-3xFLAG::Fnip1-pgk-hphPiggyBac backbone, CAG promoter driving murine 3xFLAG-Fnip1 fusion, Hygromycin selection
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48759pPy-CAG-GFP-IRES-PacCAG promoter driving GFP-IRES-Puromycin Acetyl Transferase
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48760pPy-CAG-Cre::ERT2-IRES-BSDCAG promoter driving Cre-ERT2 fusion-IRES-Blasticidin resistance
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Antibodies from Article