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PurposeExpression of FLAG tagged Omomyc in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 113168 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCßS/FLAG
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Backbone manufacturerMichael Cole
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOmomyc
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SpeciesH. sapiens (human)
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MutationE57T, E64I, R70Q, R71N
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Entrez GeneMYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCbs FlagOmomyc was a gift from Sergio Nasi (Addgene plasmid # 113168 ; http://n2t.net/addgene:113168 ; RRID:Addgene_113168) -
For your References section:
The action mechanism of the Myc inhibitor termed Omomyc may give clues on how to target Myc for cancer therapy. Savino M, Annibali D, Carucci N, Favuzzi E, Cole MD, Evan GI, Soucek L, Nasi S. PLoS One. 2011;6(7):e22284. doi: 10.1371/journal.pone.0022284. Epub 2011 Jul 21. 10.1371/journal.pone.0022284 PubMed 21811581
Map uploaded by the depositor.
