PurposeRed fluorescent glutamate sensor based on cpmApple. Membrane-displayed
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||107335||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5268
- Total vector size (bp) 6819
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Alt namecpmApple based glutamate sensor
Insert Size (bp)1641
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Please note that mutation M178K in the Neomycin resistance cassette was found during Addgene's quality control. Neomycin selection has not been tested for this plasmid, but should not affect sensor function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDisplay-R-iGluSnFR1 was a gift from Robert Campbell (Addgene plasmid # 107335 ; http://n2t.net/addgene:107335 ; RRID:Addgene_107335)
For your References section:Genetically Encoded Glutamate Indicators with Altered Color and Topology. Wu J, Abdelfattah AS, Zhou H, Ruangkittisakul A, Qian Y, Ballanyi K, Campbell RE. ACS Chem Biol. 2018 Jan 22. doi: 10.1021/acschembio.7b01085. 10.1021/acschembio.7b01085 PubMed 29308878