159,237 results

1. pcDNA3.3_SOX2

   Plasmid

   #26817

   Depositor

   Derrick Rossi

   Article

   Warren et al Cell Stem Cell. 2010 Sep 29. ():.

   Insert

   5'UTR-SOX2-3'UTR (SOX2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. C1-F-tractin-mCitrine

   Plasmid

   #155217

   Purpose

   Monitor global F-actin distribution

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine (Itpka Rat)
   

   Use

   Tags

   mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVITRO1-mCardinal-CAXX

   Plasmid

   #184451

   Purpose

   Plasma membrane-targeted mCardinal expression in mammalian cells

   Depositor

   Chuan-Hsiang Huang

   Article

   Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

   Insert

   mCardinal-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Rat EF1α

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-jAspSnFR3-mRuby3

   Plasmid

   #203498

   Purpose

   jAspSnFR3-mRuby3 in lentiviral expression vector

   Depositor

   Lucas Sullivan

   Article

   Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

   Insert

   jAspSnFR3-mRuby3
   

   Use

   Lentiviral

   Tags

   His-tag and mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFJ1320

   Plasmid

   #84829

   Purpose

   Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-2 introns

   Depositor

   Andrew Fire

   Article

   Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

   Insert

   C. elegans codon optimized GFP
   

   Use

   Tags

   Expression

   Bacterial and Worm

   Mutation

   Promoter

   Peft-3

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGWB445

   Plasmid

   #74831

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF2347

   Plasmid

   #144014

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   HOXD8 (HOXD8 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. ALCAM-p2A-E2Crimson

   Plasmid

   #83939

   Purpose

   Lentiviral vector expressing ALCAM-p2A-E2Crimson

   Depositor

   David Sabatini

   Article

   Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

   Insert

   ALCAM (ALCAM Human)
   

   Use

   Lentiviral

   Tags

   p2A-E2Crimson

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXN22-Dest

   Plasmid

   #105085

   Purpose

   Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

   Depositor

   Christopher Grefen

   Article

   Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

   Type

   Empty backbone

   Use

   Tags

   NubG-3xHA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENN.AAV.CMVs.TurboRFP.WPRE.RBG

    Plasmid

    #105548

    Purpose

    AAV expression of TurboRFP from CMV promoter

    Depositor

    James M. Wilson

    Has Service

    AAV1 and AAV8

    Insert

    TurboRFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG1242 NLS-mCherry-FIREtag

    Plasmid

    #205170

    Purpose

    Expresses NLS-mCherry-FIREtag in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    NLS-mCherry-FIREtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. p8266 LentiCRISPR v2 hygro sgNT-1

    Plasmid

    #193977

    Purpose

    Expression of spCas9 and non-targeting control sgRNA

    Depositor

    Elizabeth White

    Article

    Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

    Insert

    spCas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GCGR-DuET

    Plasmid

    #213250

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GCGR (GCGR Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. mPlum-MyosinIIA-C-18

    Plasmid

    #55989

    Purpose

    Localization: Cytoskeleton, Excitation: 590, Emission: 649

    Depositor

    Michael Davidson

    Insert

    MyosinIIA (Myh9 Mouse)
    

    Use

    Tags

    mPlum

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV500 A-CREB

    Plasmid

    #33371

    Depositor

    Charles Vinson

    Article

    Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

    Insert

    CREB (Creb1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Dominant Negative (see notes)

    Promoter

    CMV

    Available since

    Dec. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-BirA*-dCas9-eGFP

    Plasmid

    #84779

    Purpose

    Expresses BirA*-dCas9-eGFP in mammalian cells.

    Depositor

    Heinrich Leonhardt

    Article

    Schmidtmann et al Nucleus. 2016 Sep 27:0.

    Insert

    BirA*-dCas9-eGFP
    

    Use

    CRISPR

    Tags

    cMyc

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-G-Rac1

    Plasmid

    #180575

    Purpose

    ddGFP sensor of Rac1

    Depositor

    Won Do Heo

    Article

    Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

    Insert

    ddGFP sensor of Rac1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. PRDM1

    Plasmid

    #25249

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PRDM1_04 (PRDM1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

19. AiP11851-pAAV-hDLX-minBG-iCre-4X2C-WPRE3-BGHpA (Alias: CN1851)

    Plasmid

    #164450

    Purpose

    AAV vector for Cre expression in forebrain GABAergic interneurons. For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors.

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

    Has Service

    AAV PHP.eB

    Insert

    iCre
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBetaGlobin

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. DYRK1A/pTRE3G-FL-hXIST

    Plasmid

    #149608

    Purpose

    Express doxycycline-inducible full length of human XIST cDNA

    Depositor

    Jeanne Lawrence

    Article

    Jiang et al Nature. 2013 Aug 15;500(7462):296-300. d

    Insert

    X-inactive specific transcript (XIST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE3G

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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