161,570 results

1. HA Sec13 pRK5

   Plasmid

   #46332

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

   Insert

   Sec13 (SEC13 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. nls-mCherry

   Plasmid

   #108881

   Purpose

   Expression of nuclear localized mCherry

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   nls-mCherry
   

   Use

   Can be used for mammalian expression or in vitro …

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF0793

   Plasmid

   #142692

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ATF5 (ATF5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_hSyn1-SIO-stGtACR2-FusionRed

   Plasmid

   #105677

   Purpose

   Expresses a soma-targeted GtACR2 in Cre-expressing neurons

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

   Has Service

   AAV Retrograde, AAV1, and AAV5

   Insert

   stGtACR2
   

   Use

   AAV

   Tags

   FusionRed and Kv2.1

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   Feb. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1-Flag-SmBiT-PP1b

   Plasmid

   #224895

   Purpose

   Mammalian expression of Flag-SmBiT-PP1b

   Depositor

   Mathieu Bollen

   Article

   Claes et al Cell Chem Biol. 2023 Dec 21;30(12):1666-

   Insert

   Flag-SmBiT-PP1b
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiCRISPR-v2-HygR-mCherry

   Plasmid

   #167189

   Purpose

   Lentiviral expression of sgRNA with hygromycin resistance gene and mCherry

   Depositor

   Yin Shen

   Article

   Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

   Insert

   Hygromycin B phosphotransferase and mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG CMV N-HA Puro

   Plasmid

   #237879

   Purpose

   Empty mammalian expression plasmid for cloning N-terminally HA-tagged proteins

   Depositor

   Meghan Rego, Addgene Research Program

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 12, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pUC19_msfGFP-EWSR1-repair-tempate

   Plasmid

   #237682

   Purpose

   For knock-in msfGFP to EWSR1 locus

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   CRISPR

   Tags

   msfGFP

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX458_mCherry-EWSR1-gRNA

   Plasmid

   #237683

   Purpose

   Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to EWSR1 locus

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   EWSR1 gRNA (EWSR1 Human)
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV Jph3gRNA mEmerald hSyn mTagBFP2-CAAX2

    Plasmid

    #236246

    Purpose

    Endogenous tagging of Junctophilin 3 with mEmerald with a fluorescent marker for the plasma membrane

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    gRNA for rat Jph3, mEmerald donor and mTagBFP2-CAAX2
    

    Use

    AAV

    Tags

    mTagBFP2

    Expression

    Mutation

    Promoter

    U6 and human Synapsin1

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAX1_HS_gpN:SnoopTag

    Plasmid

    #225195

    Purpose

    Used for adding C-terminal SnoopTag to the E. coli HS P2 phage major capsid protein

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    gpN homology arms and SnoopTag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Enterovirus A71 (EV-A71) isolate TW-2553-1998 3C protease

    Plasmid

    #228636

    Purpose

    Bacterial expression of the A71 3C protease (isoform B) used in crystallisation

    Depositor

    Frank von Delft, ASAP Discovery

    Insert

    Enterovirus A71 3C protease isoform B
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pCAGGS puro human-GFP-RLIM-IRES-mCherry

    Plasmid

    #232243

    Purpose

    Mammalian expression of RLIM-GFP-IRES-mCherry

    Depositor

    Francisco Bustos

    Article

    Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

    Insert

    RLIM-GFP-IRES-mCherry (RLIM Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS puro human-GFP-N581K RLIM-IRES-mCherry

    Plasmid

    #232244

    Purpose

    Mammalian expression of N581K RLIM-GFP-IRES-mCherry

    Depositor

    Francisco Bustos

    Article

    Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

    Insert

    N581K RLIM-GFP-IRES-mCherry (RLIM Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    N581K

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS puro GFP-REX1[degron]-IRES-mCherry

    Plasmid

    #232240

    Purpose

    Mammalian expression of REX1[degron]-GFP-IRES-mCherry

    Depositor

    Francisco Bustos

    Article

    Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

    Insert

    REX1[degron]-GFP-IRES-mCherry (Zfp42 Mouse)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    REX1 (aa 70-85)

    Promoter

    Available since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPB1163

    Plasmid

    #210032

    Purpose

    Contains Cas3 gRNA cloning site, inducible Cas11-P2A-Cas6-T2A-Cas3, rtTA-T2A-blast

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas11-P2A-Cas6-T2A-Cas3
    rtta-T2A-blast
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. CA-0192 CMVTO-Renin(mouse)-ECL-CD4-tevs-RXR6.2

    Plasmid

    #231204

    Purpose

    ER-retained mouse renin

    Depositor

    Xiaojing Gao

    Article

    Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

    Insert

    Renin(mouse)-ECL-CD4-tevs-RXR6.2 (Ren1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. CA-0193 CMVTO-Renin(H2M)-ECL-CD4-tevs-RXR6.2

    Plasmid

    #231203

    Purpose

    ER-retained H2M renin

    Depositor

    Xiaojing Gao

    Article

    Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

    Insert

    Renin(H2M)-ECL-CD4-tevs-RXR6.2 (REN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L147I, R148H, I203V, L290V

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-30nmEML-mRFP

    Plasmid

    #231182

    Purpose

    Expresses a synthetic linker, tagged with mRFP, stabilizing the ER-mitochondrial distance at 30 nm from a lentiviral vector

    Depositor

    Dmitry Lim

    Article

    Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

    Insert

    30nmEML-mRFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV syn Barcode1

    Plasmid

    #229062

    Purpose

    Expression mapping

    Depositor

    Scott Sternson

    Insert

    Syn Barcode1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge