Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

Lysine 27 ubiquitination of the mitochondrial transport protein Miro is dependent on serine 65 of the Parkin ubiquitin ligase.

Birsa N, Norkett R, Wauer T, Mevissen TE, Wu HC, Foltynie T, Bhatia K, Hirst WD, Komander D, Plun-Favreau H, Kittler JT
J Biol Chem. 2014 May 23;289(21):14569-82. doi: 10.1074/jbc.M114.563031. Epub 2014 Mar 26. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
121151pRK5-HA-Ubiquitin-K6Mammalian expression of HA tagged ubiquitin with K6 only, other lysines mutated to arginines
121152pRK5-HA-Ubiquitin-K11Mammalian expression of HA tagged ubiquitin with K11 only, other lysines mutated to arginines
121153pRK5-HA-Ubiquitin-K6RMammalian expression of HA tagged ubiquitin K6R
121154pRK5-HA-Ubiquitin-K11RMammalian expression of HA tagged ubiquitin K11R
121155pRK5-HA-Ubiquitin-K27RMammalian expression of HA tagged ubiquitin K27R
121156pRK5-HA-Ubiquitin-K27R-K29RMammalian expression of HA tagged ubiquitin K27R and K29R
127588GFP-Miro1 S156APhospho-mutant of S156
127589GFP-Miro1 S156EPhospho-mimetic of S156
127621GFP-Trak1N-terminal GFP-tagged mouse Trak1
127622GFP-Trak2N-terminal GFP-tagged mouse Trak2

Antibodies from Article