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Lysine 27 ubiquitination of the mitochondrial transport protein Miro is dependent on serine 65 of the Parkin ubiquitin ligase.
Birsa N, Norkett R, Wauer T, Mevissen TE, Wu HC, Foltynie T, Bhatia K, Hirst WD, Komander D, Plun-Favreau H, Kittler JT
J Biol Chem. 2014 May 23;289(21):14569-82. doi: 10.1074/jbc.M114.563031. Epub 2014 Mar 26.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
121151pRK5-HA-Ubiquitin-K6Mammalian expression of HA tagged ubiquitin with K6 only, other lysines mutated to arginines
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121152pRK5-HA-Ubiquitin-K11Mammalian expression of HA tagged ubiquitin with K11 only, other lysines mutated to arginines
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121153pRK5-HA-Ubiquitin-K6RMammalian expression of HA tagged ubiquitin K6R
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121154pRK5-HA-Ubiquitin-K11RMammalian expression of HA tagged ubiquitin K11R
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121155pRK5-HA-Ubiquitin-K27RMammalian expression of HA tagged ubiquitin K27R
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121156pRK5-HA-Ubiquitin-K27R-K29RMammalian expression of HA tagged ubiquitin K27R and K29R
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127588GFP-Miro1 S156APhospho-mutant of S156
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127589GFP-Miro1 S156EPhospho-mimetic of S156
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127621GFP-Trak1N-terminal GFP-tagged mouse Trak1
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127622GFP-Trak2N-terminal GFP-tagged mouse Trak2
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