pRK5-HA-Ubiquitin-K11R
(Plasmid #121154)

• Purpose: Mammalian expression of HA tagged ubiquitin K11R
• Depositing Lab: Josef Kittler
• Publication: Birsa et al J Biol Chem. 2014 May 23;289(21):14569-82. doi: 10.1074/jbc.M114.563031. Epub 2014 Mar 26.

Backbone

• Vector backbone: pRK5-HA
• Backbone manufacturer: Ted Dawson, Addgene plasmid #17608
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ubiquitin C
• Alt name: Ub
• Species: H. sapiens (human)
• Mutation: K11 mutated to an R
• Entrez Gene: UBC (a.k.a. HMG20)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry