M30: pHR-SFFVp- HNRNPA1C::mCherry::SspB
PurposeC terminal domain of hnRNPA1 (186-320) tagged with SspB and mCherry.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||122668||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 9000
- Total vector size (bp) 10550
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)405
MutationContains only amino acids 186-230
Entrez GeneHNRNPA1 (a.k.a. ALS19, ALS20, HNRPA1, HNRPA1L3, IBMPFD3, UP 1, hnRNP A1, hnRNP-A1)
- Promoter SFFV
/ Fusion Protein
- mCherry-SspB (C terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
Vector contains SspB, a bacterial protein which binds exposed SsrA tag (from iLID) at high affinity.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:M30: pHR-SFFVp- HNRNPA1C::mCherry::SspB was a gift from Clifford Brangwynne (Addgene plasmid # 122668 ; http://n2t.net/addgene:122668 ; RRID:Addgene_122668)
For your References section:Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds. Bracha D, Walls MT, Wei MT, Zhu L, Kurian M, Avalos JL, Toettcher JE, Brangwynne CP. Cell. 2018 Nov 29;175(6):1467-1480.e13. doi: 10.1016/j.cell.2018.10.048. 10.1016/j.cell.2018.10.048 PubMed 30500534