M31: pHR-SFFVp- TDP-43C::mCherry::SspB
(Plasmid #122669)

• Purpose: C terminal domain of TDP43 (218-414) tagged with SspB and mCherry.
• Depositing Lab: Clifford Brangwynne
• Publication: Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13. doi: 10.1016/j.cell.2018.10.048.

Backbone

• Vector backbone: pHR
• Backbone size w/o insert (bp): 9000
• Total vector size (bp): 10730
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TDP43
• Species: H. sapiens (human)
• Insert Size (bp): 591
• Mutation: Contains only amino acids 218-414
• Entrez Gene: TARDBP (a.k.a. ALS10, TDP-43)
• Promoter: SFFV
• Tag / Fusion Protein:
  • mCherry-SspB (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Vector contains SspB, a bacterial protein which binds exposed SsrA tag (from iLID) at high affinity.