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M31: pHR-SFFVp- TDP-43C::mCherry::SspB
(Plasmid #122669)


Item Catalog # Description Quantity Price (USD)
Plasmid 122669 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 9000
  • Total vector size (bp) 10730
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Contains only amino acids 218-414
  • Entrez Gene
    TARDBP (a.k.a. ALS10, TDP-43)
  • Promoter SFFV
  • Tag / Fusion Protein
    • mCherry-SspB (C terminal on insert)

Cloning Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Vector contains SspB, a bacterial protein which binds exposed SsrA tag (from iLID) at high affinity.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    M31: pHR-SFFVp- TDP-43C::mCherry::SspB was a gift from Clifford Brangwynne (Addgene plasmid # 122669 ; ; RRID:Addgene_122669)
  • For your References section:

    Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds. Bracha D, Walls MT, Wei MT, Zhu L, Kurian M, Avalos JL, Toettcher JE, Brangwynne CP. Cell. 2018 Nov 29;175(6):1467-1480.e13. doi: 10.1016/j.cell.2018.10.048. 10.1016/j.cell.2018.10.048 PubMed 30500534