lenti F9-TetR-EGFP-IRES-PuroR
(Plasmid #117049)

• Purpose: lentiviral vector for expression of TetR-EGFP, for visualization of TetO repeats
• Depositing Lab: Huimin Zhao
• Publication: Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 5038283. doi: 10.1093/nar/gky501.

Backbone

• Vector backbone: Cre-IRES-PuroR
• Backbone manufacturer: Addgene Plasmid #30205
• Backbone size w/o insert (bp): 7177
• Total vector size (bp): 9451
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Growth in a bacterial strain that is suitable for repetitive sequences is recommended.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: F9-TetR-EGFP
• Insert Size (bp): 2274
• Promoter: F9 promoter
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SpeI (not destroyed)
• 3′ cloning site: PshAI (not destroyed)
• 5′ sequencing primer: GAGGCATTGTAGAGGCTTC and AGGTCGACTCTAGACGATC
• 3′ sequencing primer: IRES-R

Resource Information

• Supplemental Documents:
  • lenti F9-tetR-EGFP-ires-Pur.gb
• A portion of this plasmid was derived from a plasmid made by: F9-TetR-EGFP sequence was obtained from Dr. Andrew Belmont.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry