PurposeCry1 transcription fluorescence reporter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||110054||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4171
- Total vector size (bp) 7688
Vector typeMammalian Expression, AAV
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameCry1 promoter and Venus
Alt nameCry1 intron336
SpeciesM. musculus (mouse)
Entrez GeneCry1 (a.k.a. AU020726, AU021000, Ph, Phll1)
- Promoter Cry1
/ Fusion Protein
- NLS-D2 (C terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
This plasmid was generated based on a plasmid from the laboratory of Hiroki Ueda. Please also include the following citation when using this plasmid: Delay in Feedback Repression by Cryptochrome 1 Is Required for Circadian Clock Function. Ukai-Tadenuma, Maki et al. Cell, Volume 144, Issue 2, 268 – 281.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AAV-P(Cry1)-forward-intron336-Venus-NLS-D2 was a gift from Eric Erquan Zhang (Addgene plasmid # 110054 ; http://n2t.net/addgene:110054 ; RRID:Addgene_110054)
For your References section:Long-term in vivo recording of circadian rhythms in brains of freely moving mice. Mei L, Fan Y, Lv X, Welsh DK, Zhan C, Zhang EE. Proc Natl Acad Sci U S A. 2018 Apr 2. pii: 1717735115. doi: 10.1073/pnas.1717735115. 10.1073/pnas.1717735115 PubMed 29610316