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AAV-P(Cry1)-DIO-intron336-Venus-NLS-D2
(Plasmid #110055)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 110055 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    aav2
  • Backbone size w/o insert (bp) 4171
  • Total vector size (bp) 7683
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cry1 promoter and Venus
  • Alt name
    Cry1 intron336
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Cry1 (a.k.a. Phll1)
  • Promoter Cry1
  • Tag / Fusion Protein
    • NLS-D2 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was generated based on a plasmid from the laboratory of Hiroki Ueda. Please also include the following citation when using this plasmid: Delay in Feedback Repression by Cryptochrome 1 Is Required for Circadian Clock Function. Ukai-Tadenuma, Maki et al. Cell, Volume 144, Issue 2, 268 – 281.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-P(Cry1)-DIO-intron336-Venus-NLS-D2 was a gift from Eric Erquan Zhang (Addgene plasmid # 110055 ; http://n2t.net/addgene:110055 ; RRID:Addgene_110055)
  • For your References section:

    Long-term in vivo recording of circadian rhythms in brains of freely moving mice. Mei L, Fan Y, Lv X, Welsh DK, Zhan C, Zhang EE. Proc Natl Acad Sci U S A. 2018 Apr 2. pii: 1717735115. doi: 10.1073/pnas.1717735115. 10.1073/pnas.1717735115 PubMed 29610316