Massively parallel assessment of human variants with base editor screens.
Hanna RE, Hegde M, Fagre CR, DeWeirdt PC, Sangree AK, Szegletes Z, Griffith A, Feeley MN, Sanson KR, Baidi Y, Koblan LW, Liu DR, Neal JT, Doench JG
Cell. 2021 Feb 18;184(4):1064-1080.e20. doi: 10.1016/j.cell.2021.01.012. PubMed Article
Hanna RE, Hegde M, Fagre CR, DeWeirdt PC, Sangree AK, Szegletes Z, Griffith A, Feeley MN, Sanson KR, Baidi Y, Koblan LW, Liu DR, Neal JT, Doench JG
Cell. 2021 Feb 18;184(4):1064-1080.e20. doi: 10.1016/j.cell.2021.01.012. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 158579 | pMT_025 | Lentiviral expression of a customizable open reading frame |
| 158580 | pLX_313-EGFP | Lentiviral expression of EGFP |
| 158581 | pRDA_256 | Lentiviral expression of BE3.9max and customizable gRNA |
| 158582 | pRDA_078 | Lentiviral expression of BE4max and customizable gRNA |
| 158583 | pRDA_085 | Lentiviral expression of SpCas9; P2A site provides mKate2 |