Salmonella-based platform for efficient delivery of functional binding proteins to the cytosol.
Chabloz A, Schaefer JV, Kozieradzki I, Cronin SJF, Strebinger D, Macaluso F, Wald J, Rabbitts TH, Pluckthun A, Marlovits TC, Penninger JM
Commun Biol. 2020 Jul 3;3(1):342. doi: 10.1038/s42003-020-1072-4. PubMed Article
Chabloz A, Schaefer JV, Kozieradzki I, Cronin SJF, Strebinger D, Macaluso F, Wald J, Rabbitts TH, Pluckthun A, Marlovits TC, Penninger JM
Commun Biol. 2020 Jul 3;3(1):342. doi: 10.1038/s42003-020-1072-4. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 153322 | pCASP-SptP167-3G124-HilA | Triggers secretion of SptP167 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose |
| 153323 | pCASP-SptP120-3G124-HilA | Triggers secretion of SptP120 fused to the 3G124nc anti-GFP DARPin from Salmonella upon induction with arabinose |
| 153324 | pCASP-3G124-HilA | Shows that expression of the protein of interest from Salmonella is disrupted when SptP signal is removed from the pCASP-HilA vector |
| 153325 | pCASP-SptP120-3m160-HilA | Triggers secretion of SptP120 fused to the 3m160 anti-mCherry DARPin from Salmonella upon induction with arabinose |
| 153326 | pCASP-SptP120-E3_5-HilA | Triggers secretion of SptP120 fused to the E3_5 DARPin from Salmonella upon induction with arabinose |
| 153327 | pCASP-SptP120-NS1-HilA | Triggers secretion of SptP120 fused to the NS1 monobody from Salmonella upon induction with arabinose |
| 153328 | pCASP-SptP120-K27-HilA | Triggers secretion of SptP120 fused to the K27 DARPin from Salmonella upon induction with arabinose |
| 153329 | pCASP-SptP120-K55-HilA | Triggers secretion of SptP120 fused to the K55 DARPin from Salmonella upon induction with arabinose |
| 153330 | pCASP-SptP120-TD-HilA | Triggers secretion of SptP120 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose |
| 153331 | pCASP-SptP92-TD-HilA | Triggers secretion of SptP92 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose |
| 153332 | pCASP-SptP35-TD-HilA | Triggers expression of SptP35 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose |
| 153333 | pCASP-HilA empty vector | Triggers HilA overexpression from Salmonella upon induction with arabinose without any synthetic protein expressed |
| 153335 | Lenti-mCherry-WT-KRAS-IRES-Blast | Lentiviral vector plasmid for integration and constitutive expression of mCherry fused to wild-type KRAS in mammalian cells |
| 153336 | Lenti-mCherry-G12V-KRAS-IRES-Blast | Lentiviral vector plasmid for integration and constitutive expression of mCherry fused to oncogenic G12V-KRAS in mammalian cells |
| 154009 | Lenti-AcGFP-Sec61-IRES-Blast | Lentiviral vector plasmid for integration and constitutive expression of AcGFP fused to Sec61 in mammalian cells – gift from D. Gerlich (IMBA, Vienna) |