163,504 results

1. CIRTS-6: bdefensin 3-TBP6.7-YTHDF2(1-200)

   Plasmid

   #132544

   Purpose

   Expresses CIRTS-6 (bdefensin 3-TBP6.7-YTHDF2(1-200)) in mammalian cells

   Depositor

   Bryan Dickinson

   Article

   Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

   Insert

   CIRTS-6: bdefensin 3-TBP6.7-YTHDF2(1-200)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   cmv d0

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-FLAG-hHelios-IRES-GFP

   Plasmid

   #74047

   Purpose

   retroviral plasmid for expression of FLAG-tagged human Helios (IKZF2) corresponding to cDNA from NM_001079526.1

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   human Helios (IKZF2) (IKZF2 )
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   encodes shorter version of human Helios

   Promoter

   pMSCV-LTRs

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-Rab11-WT

   Plasmid

   #101047

   Purpose

   Expresses HA-tagged human Rab11-WT in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Article

   Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

   Insert

   RAB11 (RAB11A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. MAC_C_LTK

   Plasmid

   #187762

   Purpose

   MAC-tagged gene expression of human LTK

   Depositor

   Markku Varjosalo

   Article

   Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

   Insert

   LTK (LTK Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. lenti F9-TetR-EGFP-IRES-PuroR

   Plasmid

   #117049

   Purpose

   lentiviral vector for expression of TetR-EGFP, for visualization of TetO repeats

   Depositor

   Huimin Zhao

   Article

   Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

   Insert

   F9-TetR-EGFP
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   F9 promoter

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. IMS-APEX2

   Plasmid

   #79058

   Purpose

   Expresses APEX in the mitochondrial intermembrane space.

   Depositor

   Alice Ting

   Article

   Hung et al Mol Cell. 2014 Jul 17;55(2):332-41. doi:

   Insert

   APEX
   

   Use

   Tags

   V5

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7 promoter

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-BRD3

   Plasmid

   #65377

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BRD3 (BRD3 Human)
   

   Use

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV pEF1a-DIO-FLPo-WPRE-hGHpA

   Plasmid

   #87306

   Purpose

   An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

   Depositor

   Li Zhang

   Article

   Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   Cre-dependent mouse codon-optimized Flp recombinase.
   

   Use

   AAV

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJPT002-M-HaloTag-1x FLAG

   Plasmid

   #202493

   Purpose

   Express "M" HaloTag

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

   Insert

   HaloTag
   

   Use

   Tags

   1x FLAG and Myristoylation site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWB417

    Plasmid

    #74811

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available Since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-Cre-iBSD

    Plasmid

    #140284

    Purpose

    Expression of Cre-IRES-BSD

    Depositor

    Yusuke Miyanari

    Article

    Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

    Insert

    Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. BirA(R118G)-HA destination vector

    Plasmid

    #53581

    Purpose

    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)

    Depositor

    Karl Kramer

    Type

    Empty backbone

    Use

    Tags

    BirA(R118G) and HA tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSET-iGluSnFR4f

    Plasmid

    #234444

    Purpose

    Bacterial expression of glutamate sensor with with improved sensitivity and fast deactivation kinetics (4f = fast)

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f
    

    Use

    Tags

    6xHis-Xpress epi

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA

    Plasmid

    #196192

    Purpose

    human synapsin promoter driven dTomato expression at human AAVS1 safe harbour locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
    

    Use

    Znf donor

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-nSpCas9-evoCDA1

    Plasmid

    #209042

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    evoCDA1-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Perox-RA

    Plasmid

    #209869

    Purpose

    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Use

    Tags

    Targeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBeta-lacZ-attBx2

    Plasmid

    #124445

    Purpose

    Plasmid for in vivo enhancer reporter assay. Plasmid contains a β-globin minimal promoter followed by a lacZ reporter gene derived from pRS16, with the entire reporter cassette flanked by attB sites.

    Depositor

    Yingguang Frank Chan

    Article

    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

    Type

    Empty backbone

    Use

    Enhancer reporter assay construct

    Tags

    LacZ

    Expression

    Mammalian

    Mutation

    Promoter

    beta globin minimal promoter

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-MAPK1

    Plasmid

    #116760

    Purpose

    Lentiviral expression of MAPK1

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. mApple-Alpha-V-Integrin-25

    Plasmid

    #54866

    Purpose

    Localization: Focal Adhesions, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    Alpha-V Integrin (ITGAV Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti6.3-sRGN3.1-BlastR

    Plasmid

    #220964

    Purpose

    Expresses human codon-optimized sRGN3.1

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    sRGN3.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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