159,237 results

1. pCas9D10A_GFP

   Plasmid

   #44720

   Purpose

   Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Cas9D10A-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Mutation

   D10A mutation- nickase mutant

   Promoter

   CAG

   Available since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pChillyBoys_gen1

   Plasmid

   #228495

   Purpose

   pACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)

   Depositor

   Richard Baker

   Inserts

   groEL
   groES
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRE-Tn5-132

   Plasmid

   #118518

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sGFP2
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLEX307 ASC

   Plasmid

   #163977

   Purpose

   Mammalian expression vector encoding ASC (for example, to assess ASC-speck formation or inflammasome signalling assays)

   Depositor

   Daniel Bachovchin

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   ASC (PYCARD Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cas12f-GE ver4.1

   Plasmid

   #176544

   Purpose

   Expresses Cas12f-GE in mammalian cells

   Depositor

   Yong-Sam Kim

   Article

   Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

   Inserts

   Cas12f-GE ver4.1
   Cas12f-GE ver4.1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and chicken β-actin

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-C1-hAtg13

   Plasmid

   #22875

   Depositor

   Noboru Mizushima

   Article

   Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

   Insert

   autophagy-related genes 13 (ATG13 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3 Flag TSC2

   Plasmid

   #14129

   Depositor

   Brendan Manning

   Article

   Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

   Insert

   TSC2 (TSC2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pICE-EGFP-FLAG-PLIN3

   Plasmid

   #161918

   Purpose

   Expresses human PLIN3 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   Perilipin-3 (PLIN3 Human)
   

   Use

   Tags

   GFP-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-puro-Bcl-XL

   Plasmid

   #46972

   Purpose

   lentiviral expression of Bcl-XL

   Depositor

   Jialiang Wang

   Article

   Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

   Insert

   BCL2L1 (BCL2L1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET22B_SA_PcrA

    Plasmid

    #102999

    Purpose

    The plasmid is used to express Staphylococcus aureus PcrA, a DNA helicase, with N-terminal His-tag and Gly-Gly linker

    Depositor

    Martin Webb

    Insert

    SA_PcrA
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    PcrA 199-2423

    Promoter

    T7

    Available since

    May 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-NLS-tdMCP-eGFP

    Plasmid

    #86243

    Purpose

    Middle entry vector for inserting promotors for tissue specific expression of NLS-tdMCP-eGFP reporter

    Depositor

    Florence Marlow

    Article

    Campbell et al Development. 2015 Apr 1;142(7):1368-74.

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    NLS-tdMCP-eGFP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

    Plasmid

    #201916

    Purpose

    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Inserts

    Gag-Cas9 v2
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and Human U6

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLTR.gp140/EGFP.Revdelta38/DsRed

    Plasmid

    #115775

    Purpose

    HIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.

    Depositor

    Damian Purcell

    Article

    Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

    Insert

    Envelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
    

    Use

    Tags

    EGFP; dsReD

    Expression

    Mammalian

    Mutation

    gp140 uncleaved (1190-91aa, KR > TG; truncated…

    Promoter

    HIV-1 (LTR)

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3XFlagNICD1

    Plasmid

    #20183

    Depositor

    Raphael Kopan

    Article

    Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

    Insert

    Notch 1 Intracellular Domain (Notch1 Mouse)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Contains murine Notch 1 Intracellular Domain (Val…

    Promoter

    CMV

    Available since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.3_c-MYC

    Plasmid

    #26818

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Sep 29. ():.

    Insert

    5'UTR-c-MYC-3'UTR (MYC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Sequence begins from second ATG; Synthetic UTR se…

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pcDNA3.1 Mfn2-HA

    Plasmid

    #139192

    Purpose

    Expresses C-terminal HA tagged wt human Mfn2 in mammalian cells

    Depositor

    Allan Weissman

    Article

    Leboucher et al Mol Cell. 2012 Aug 24;47(4):547-57. doi:

    Insert

    MFN2 (MFN2 Human)
    

    Use

    Tags

    3HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFPC2-Gephyrin P1

    Plasmid

    #68815

    Purpose

    expression of fluorescent tagged Geph in mammalian cells

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    silent mutations on internal EcoRI site. Gephyri…

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiCMVNeoDEST(705-1)-hAXL

    Plasmid

    #124796

    Purpose

    Expresses AXL in mammalian cells

    Depositor

    Kenneth Pienta

    Article

    Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

    Insert

    AXL (AXL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pZW6 (CK2alpha)

    Plasmid

    #27086

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2alpha (CSNK2A1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-DAAO

    Plasmid

    #60344

    Purpose

    Yeast D-Amino acid oxidase from Rhodotorula gracilis, optimized for mammalian expression.

    Depositor

    Vsevolod Belousov

    Article

    Matlashov et al Antioxid Redox Signal. 2014 Mar 1;20(7):

    Insert

    DAAO
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge