Combined fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants.
Ursache R, Fujita S, Denervaud Tendon V, Geldner N
Plant Methods. 2021 Oct 30;17(1):111. doi: 10.1186/s13007-021-00811-9. PubMed Article
Ursache R, Fujita S, Denervaud Tendon V, Geldner N
Plant Methods. 2021 Oct 30;17(1):111. doi: 10.1186/s13007-021-00811-9. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 167663 | pRU41 | ENTRY vector for gRNA1 cloning under pAtU6 promoter |
| 167664 | pRU42 | ENTRY vector for gRNA2 cloning under pAtU3 promoter |
| 167665 | pRU43 | ENTRY vector for gRNA3 cloning under pAtU6 promoter |
| 167666 | pRU44 | ENTRY vector for gRNA4 cloning under pAtU3 promoter |
| 167667 | pRU45 | ENTRY vector for gRNA5 cloning under pAtU6 promoter |
| 167668 | pRU46 | ENTRY vector for gRNA6 cloning under pAtU3 promoter |
| 167669 | pRU47 | ENTRY vector for gRNA7 cloning under pAtU6 promoter |
| 167670 | pRU48 | ENTRY vector for gRNA8 cloning under pAtU3 promoter |
| 167671 | pSF463 | Intermediate vector for two gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway |
| 167672 | pSF464 | Intermediate vector for four gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway |
| 167673 | pSF278 | Intermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway |
| 167674 | pSF279 | Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway |
| 167675 | pSF280 | Intermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway |
| 167676 | pRU325 | Intermediate vector for eight gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway |
| 167677 | pRU51 | Final destination vector for single Gateway LR reaction |
| 167678 | pRU52 | Final destination vector for single Gateway LR reaction |
| 167679 | pRU53 | Final destination vector for single Gateway LR reaction |
| 167680 | pRU54 | Final destination vector for single Gateway LR reaction |
| 167681 | pRU321 | Final destination vector for single Gateway LR reaction |
| 167682 | pRU320 | Final destination vector for single Gateway LR reaction |
| 167683 | pRU322 | Final destination vector for single Gateway LR reaction |
| 167684 | pRU55 | Final destination vector for single Gateway LR reaction |
| 167685 | pRU205 | Final destination vector for single Gateway LR reaction |
| 167686 | pRU319 | Final destination vector for single Gateway LR reaction |
| 167687 | pRU292 | Final destination vector for single Gateway LR reaction |
| 167688 | pRU293 | Final destination vector for single Gateway LR reaction |
| 167689 | pRU294 | Final destination vector for single Gateway LR reaction |
| 167690 | pRU323 | Final destination vector for single Gateway LR reaction |
| 167691 | pRU324 | Final destination vector for single Gateway LR reaction |
| 167692 | pRU61 | Final destination vector for single Gateway LR reaction |
| 167693 | pRU206 | Final destination vector for single Gateway LR reaction |
| 167694 | pRU295 | Final destination vector for single Gateway LR reaction |