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pDONRp5-p2 GFP-NShroom3-iLID
(Plasmid #170976)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 170976 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 170976-D50 50 μg of DNA in Tris buffer $495
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DNA 170976-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pDONR221 p5-p2
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2553
  • Total vector size (bp) 8070
  • Vector type
    Gateway Cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NShroom3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4161
  • Mutation
    From isoform 2, deleted amino acids 1389 - 1805, mutations: G1226E, E1227K, P1242L, R1276L
  • GenBank ID
    XP_006535031.1
  • Entrez Gene
    Shroom3 (a.k.a. AL022960, D5Ertd287, D5Ertd287e, Sh, Shr, Shrm, Shrm3)
  • Tags / Fusion Proteins
    • eGFP (N terminal on insert)
    • iLID (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13 fwd: GTAAAACGACGGCCAGT
  • 3′ sequencing primer M13 rev: GTCATAGCTGTTTCCTG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Original mShroom3 sequence was a gift from T. Nishimura, from M.Takeichi lab. iLID sequence was a gift from H. Janovjak lab.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2021.04.20.440475 for bioRxiv preprint.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONRp5-p2 GFP-NShroom3-iLID was a gift from Miki Ebisuya (Addgene plasmid # 170976 ; http://n2t.net/addgene:170976 ; RRID:Addgene_170976)

  • For your References section:

    Optogenetic control of apical constriction induces synthetic morphogenesis in mammalian tissues. Martinez-Ara G, Taberner N, Takayama M, Sandaltzopoulou E, Villava CE, Bosch-Padros M, Takata N, Trepat X, Eiraku M, Ebisuya M. Nat Commun. 2022 Sep 14;13(1):5400. doi: 10.1038/s41467-022-33115-0. 10.1038/s41467-022-33115-0 PubMed 36104355
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