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(Plasmid #174726)


Item Catalog # Description Quantity Price (USD)
Plasmid 174726 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size (bp) 4506
  • Modifications to backbone
    The lacZ gene was inserted into the plasmid backbone using infusion. Two Type IIS restriction sites were added in the 3' and 5' ends of the lacZ gene. The spectinomycin resistance gene was clarified from SapI restriction sites by silent mutagenesis
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    addition of 2% x-gal to culture media is important for white blue selection
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKO403_lacZ_Sp was a gift from Tohru Suzuki (Addgene plasmid # 174726 ; ; RRID:Addgene_174726)
  • For your References section:

    Bifidobacterium-Escherichia coli Shuttle Vector Series pKO403, with Temperature-Sensitive Replication Origin for Gene Knockout in Bifidobacterium. Altaib H, Ozaki Y, Kozakai T, Sakaguchi K, Nomura I, Suzuki T. Microbiol Resour Announc. 2022 Jan 20;11(1):e0088421. doi: 10.1128/mra.00884-21. Epub 2022 Jan 13. 10.1128/mra.00884-21 PubMed 35023783