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R619-M89-303: CMV51p> HCoV-HKU1 S-2P-T4f-3C-His8-Strep2x2
(Plasmid #166014)


Item Catalog # Description Quantity Price (USD)
Plasmid 166014 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    HCoV-HKU1 S (spike)
  • Species
  • Promoter CMV51
  • Tag / Fusion Protein
    • 3C-His8-Strep2x2 (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

DNA sequence optimized for mammalian expression

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    R619-M89-303: CMV51p> HCoV-HKU1 S-2P-T4f-3C-His8-Strep2x2 was a gift from Dominic Esposito (Addgene plasmid # 166014 ; ; RRID:Addgene_166014)
  • For your References section:

    Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays. Esposito D, Mehalko J, Drew M, Snead K, Wall V, Taylor T, Frank P, Denson JP, Hong M, Gulten G, Sadtler K, Messing S, Gillette W. Protein Expr Purif. 2020 Jun 4;174:105686. doi: 10.1016/j.pep.2020.105686. 10.1016/j.pep.2020.105686 PubMed 32504802