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pInducer20 EGFP-TEADi
(Plasmid #140145)


Item Catalog # Description Quantity Price (USD)
Plasmid 140145 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Total vector size (bp) 11263
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number


  • Gene/Insert name
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For better TEAD inhibition select high expressing clones or with high dose of neomycin. We have observed that in certain cancer cell lines TEADi gets degraded or is expressed at low levels, resulting in incomplete TEAD inhibition. We recommend checking by Western Blot with anti-GFP antibody that the correct molecular weight of the full construct is expressed (approximately 39kDa) and check that known targets of TEAD are downregulated upon expression of TEADi. As with other dominant negative proteins, good levels of expression are necessary for TEADi to block TEADs.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pInducer20 EGFP-TEADi was a gift from Ramiro Iglesias-Bartolome (Addgene plasmid # 140145 ; ; RRID:Addgene_140145)
  • For your References section:

    YAP1/TAZ-TEAD transcriptional networks maintain skin homeostasis by regulating cell proliferation and limiting KLF4 activity. Yuan Y., Park J., Feng A., Awasthi P., Wang Z., Chen Q., Iglesias-Bartolome R.. Nat Commun 11, 1472 (2020). 10.1038/s41467-020-15301-0