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Depositing Lab
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Depositing OrganizationMax Planck Institute for Developmental Biology
Located in Germany -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21182 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 21182-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 21182-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepAc5.1C
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5371
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Vector typeDrosophila expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDh5 alpha or XL1-blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRenilla Luciferase
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Alt nameRLuc
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Insert Size (bp)933
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer Ac5 forward primer site
- 3′ sequencing primer BGH reverse priming site
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 21182-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 21182-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAc5.1C-RLuc-V5His6 was a gift from Elisa Izaurralde (Addgene plasmid # 21182 ; http://n2t.net/addgene:21182 ; RRID:Addgene_21182) -
For your References section:
A crucial role for GW182 and the DCP1:DCP2 decapping complex in miRNA-mediated gene silencing. Rehwinkel J, Behm-Ansmant I, Gatfield D, Izaurralde E. RNA. 2005 Nov . 11(11):1640-7. 10.1261/rna.2191905 PubMed 16177138
Map uploaded by the depositor.
