CC10-rtTA
(Plasmid #20179)

• Depositing Lab: Jeffrey Whitsett
• Publication: Stripp et al J Biol Chem. 1992 Jul 25. 267(21):14703-12.

Backbone

• Vector backbone: pBluescript II KS+
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: rcc10 promoter
• Alt name: ccsp-rtTA
• Species: R. norvegicus (rat)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7 Fwd

Resource Information

• Supplemental Documents:
  • CC10-rtTA cloning info
• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: We cloned the CC10 promoter, Tet Systems cloned the rtTA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The expression vector pCC10/NrtTA/hGH was made by ligating the following gene fragments into pBIIKS(+):
1) 2.3kb HindIII fragment of rat CC10 promoter
2) 1.0kb HindIII/BamHI fragment of NrtTA coding seq
3) 2.0kb BamHI/NotI fragment of human growth hormone gene sequence extending from the BamHI site in the first exon to the EcoRI site after the poly-A addition signal.