|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15301||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerMarc Vidal Lab
- Backbone size w/o insert (bp) 4300
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
Insert Size (bp)700
- Cloning method Gateway Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Entry vector encoding GFP that can be used as a control for LR reaction and expression experiments. The GFP is from Stratagene's hrGFP-C vector.
Please visit Invitrogen's website for detailed protocols and for LR enzyme.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pENTRY-GFP was a gift from William Hahn (Addgene plasmid # 15301 ; http://n2t.net/addgene:15301 ; RRID:Addgene_15301)
For your References section:Integrative Genomic Approaches Identify IKBKE as a Breast Cancer Oncogene. Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK, Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES, Polyak K, Hahn WC. Cell. 2007 Jun 15. 129(6):1065-1079. 10.1016/j.cell.2007.03.052 PubMed 17574021
Map uploaded by the depositor.