h3.7SP-Crtta
(Plasmid #20180)

• Depositing Lab: Jeffrey Whitsett
• Publication: Tichelaar et al J Biol Chem. 2000 Apr 21. 275(16):11858-64.

Backbone

• Vector backbone: pUC18
• Backbone manufacturer: NEB
• Backbone size w/o insert (bp): 2700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: h3.7SP-Crtta
• Alt name: Sftpc-reverse tetracycline transactivator
• Species: H. sapiens (human)
• Insert Size (bp): 5100
• Entrez Gene: SFTPC (a.k.a. BRICD6, PSP-C, SFTP2, SMDP2, SP-C, SP5)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EheI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: M13_forward20
• 3′ sequencing primer: M13rev

Resource Information

• Supplemental Documents:
  • seq info
• A portion of this plasmid was derived from a plasmid made by: We cloned the promoter (3.7SP-C), Tet Systems the rtTA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Used to express genes in the lung epithelium or lung epithelial cells