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pEGFP-C1 beta-catenin Y654E
(Plasmid #16073)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16073 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    beta-catenin
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    2345
  • Mutation
    Y654E, *(see below)
  • Entrez Gene
    CTNNB1 (a.k.a. CHBCAT)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BspE1/BamHI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Phosphorylation-mimic mutant of beta-catenin.

*Note: In addition to the described mutation, Addgene's quality control sequencing finds the following polymorphisms: L95R, S96A, G636A, and V651G.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-C1 beta-catenin Y654E was a gift from Erin Schuman (Addgene plasmid # 16073 ; http://n2t.net/addgene:16073 ; RRID:Addgene_16073)
  • For your References section:

    Depolarization drives beta-Catenin into neuronal spines promoting changes in synaptic structure and function. Murase S, Mosser E, Schuman EM. Neuron. 2002 Jul 3. 35(1):91-105. 10.1016/S0896-6273(02)00764-X PubMed 12123611