pBabe Angptl4 puro
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19156||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5169
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Growth instructionsNeed to grow in STBL3
Copy numberHigh Copy
Gene/Insert nameangiopoietin-like 4
Alt nameANGPTL4, ANGPTL2, ARP4, FIAF, HFARP, NL2, PGAR, pp1158
SpeciesH. sapiens (human)
Insert Size (bp)1200
Entrez GeneANGPTL4 (a.k.a. ARP4, FIAF, HARP, HFARP, NL2, PGAR, TGQTL, UNQ171, pp1158)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer pBABE 5'
- 3′ sequencing primer pBABE 3' (Common Sequencing Primers)
PCR out Angptl4 cDNA, used TOPO cloning kit and generated BglII sites at the ends. Cloned into BamHI site of pBabe which destroys BamHI site. Checked orientation with EcoRI digest.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBabe Angptl4 puro was a gift from Joan Massague (Addgene plasmid # 19156 ; http://n2t.net/addgene:19156 ; RRID:Addgene_19156)
For your References section:TGFbeta primes breast tumors for lung metastasis seeding through angiopoietin-like 4. Padua D, Zhang XH, Wang Q, Nadal C, Gerald WL, Gomis RR, Massague J. Cell. 2008 Apr 4. 133(1):66-77. 10.1016/j.cell.2008.01.046 PubMed 18394990
Map uploaded by the depositor.