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pRSET FLII12Pglu-700uDelta6
(Plasmid #13568)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13568 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLIPglu F16A Y12S-ECFP-D13R, 6aa linker
  • Alt name
    mglB with ECFP inserted
  • Alt name
    Periplasmic glucose binding
  • Species
    E. coli
  • Insert Size (bp)
    2400
  • Mutation
    F16A, ECFP insert in mglB
  • Tag / Fusion Protein
    • Citrine (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html

Citrine is modified version of EYFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSET FLII12Pglu-700uDelta6 was a gift from Wolf Frommer (Addgene plasmid # 13568 ; http://n2t.net/addgene:13568 ; RRID:Addgene_13568)
  • For your References section:

    GLUT1 and GLUT9 as major contributors to glucose influx in HepG2 cells identified by a high sensitivity intramolecular FRET glucose sensor. Takanaga H, Chaudhuri B, Frommer WB. Biochim Biophys Acta. 2007 Dec 14. ():. 10.1016/j.bbamem.2007.11.015 PubMed 18177733