|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15968||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerN. Buchler
- Backbone size w/o insert (bp) 5074
Vector typeBacterial Expression, Yeast Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameTEF1 promoter + polylinker + CYC1 terminator
Alt nameTranslational elongation factor EF-1 alpha
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)1060
MutationTEF1 promoter is between SacI and XbaI restriction sites.
Entrez GeneTEF1 (a.k.a. YPR080W)
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site NgoMIV (not destroyed)
- 5′ sequencing primer M13 Reverse (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p405TEF1 was a gift from Nicolas Buchler & Fred Cross (Addgene plasmid # 15968 ; http://n2t.net/addgene:15968 ; RRID:Addgene_15968)