|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15974||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerSikorski & Hieter, Genetics 122:19-27 (1989)
- Backbone size w/o insert (bp) 4283
Vector typeBacterial Expression, Yeast Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameADH1 promoter + polylinker + CYC1 terminator
Alt namealcohol dehydrogenase
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)1795
MutationADH1 promoter is between SacI and XbaI restriction sites.
Entrez GeneADH1 (a.k.a. YOL086C, ADC1)
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer M13 Reverse (Common Sequencing Primers)
Mumberg D, Muller R, Funk M. Gene 156:119-22 (1995).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p406ADH1 was a gift from Nicolas Buchler & Fred Cross (Addgene plasmid # 15974 ; http://n2t.net/addgene:15974 ; RRID:Addgene_15974)