p404TEF1
(Plasmid #15972)

• Depositing Labs: Nicolas Buchler, Fred Cross
• Publication: Buchler lab plasmids (unpublished)

Backbone

• Vector backbone: pRS404
• Backbone manufacturer: Sikorski & Hieter, Genetics 122:19-27 (1989)
• Backbone size w/o insert (bp): 4173
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TEF1 promoter + polylinker + CYC1 terminator
• Alt name: Translational elongation factor EF-1 alpha
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 728
• Mutation: TEF1 promoter is between SacI and XbaI restriction sites.
• Entrez Gene: TEF1 (a.k.a. YPR080W)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: M13 Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Plasmid was made by Ben Timney (Rockefeller University). Insert was cut from Mumberg plasmid p413TEF, which was obtained from ATCC.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mumberg D, Muller R, Funk M. Gene 156:119-22 (1995).