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Depositing Lab
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Depositing OrganizationUniversity of Massachusetts, Amherst
Located in United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18820 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 18820-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 18820-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepFA6a-kanMX6
- Backbone size w/o insert (bp) 3944
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Vector typeYeast tagging
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePhotoactivatable Green Fluorescent Protein
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Alt namePA-GFP
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Alt namePhotoactivatable GFP
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SpeciesAequorea victoria
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Insert Size (bp)720
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer CGATTTAGGTGACACTAT
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byPatricia Wadsworth, University of Massachusetts Amherst (pIREShyg-PA-GFP-alpha Tubulin)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
U.S. Tulu, et al. (2003). "Peripheral, Non-Centrosome-Associated Microtubules Contribute to Spindle Formation in Centrosome-Containing Cells" Curr Biol 13: 1894-99.
DNA (Catalog # 18820-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 18820-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6a-PA-GFP-kanMX6 was a gift from Wei-Lih Lee (Addgene plasmid # 18820 ; http://n2t.net/addgene:18820 ; RRID:Addgene_18820) -
For your References section:
Photoactivatable GFP tagging cassettes for protein-tracking studies in the budding yeast Saccharomyces cerevisiae. Vorvis C, Markus SM, Lee WL. Yeast. 2008 Sep;25(9):651-9. 10.1002/yea.1611 PubMed 18727145
