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(Plasmid #18822)


Item Catalog # Description Quantity Price (USD)
Plasmid 18822 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 3831
  • Vector type
    Yeast tagging
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Photoactivatable Green Fluorescent Protein
  • Alt name
  • Alt name
    Photoactivatable GFP
  • Species
    Aequorea victoria
  • Insert Size (bp)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Patricia Wadsworth, University of Massachusetts Amherst (pIREShyg-PA-GFP-alpha Tubulin)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

U.S. Tulu, et al. (2003). "Peripheral, Non-Centrosome-Associated Microtubules Contribute to Spindle Formation in Centrosome-Containing Cells" Curr Biol 13: 1894-99.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-PA-GFP-His3MX6 was a gift from Wei-Lih Lee (Addgene plasmid # 18822 ; ; RRID:Addgene_18822)
  • For your References section:

    Photoactivatable GFP tagging cassettes for protein-tracking studies in the budding yeast Saccharomyces cerevisiae. Vorvis C, Markus SM, Lee WL. Yeast. 2008 Sep;25(9):651-9. 10.1002/yea.1611 PubMed 18727145