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pFA6a-PA-GFP-TRP1
(Plasmid #18821)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18821 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFA6a-TRP1
  • Backbone size w/o insert (bp) 3421
  • Vector type
    Yeast tagging
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Photoactivatable Green Fluorescent Protein
  • Alt name
    PA-GFP
  • Alt name
    Photoactivatable GFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    720

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer CGATTTAGGTGACACTAT
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Patricia Wadsworth, University of Massachusetts Amherst (pIREShyg-PA-GFP-alpha Tubulin)

Terms and Licenses

Depositor Comments

U.S. Tulu, et al. (2003). "Peripheral, Non-Centrosome-Associated Microtubules Contribute to Spindle Formation in Centrosome-Containing Cells" Curr Biol 13: 1894-99.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-PA-GFP-TRP1 was a gift from Wei-Lih Lee (Addgene plasmid # 18821 ; http://n2t.net/addgene:18821 ; RRID:Addgene_18821)
  • For your References section:

    Photoactivatable GFP tagging cassettes for protein-tracking studies in the budding yeast Saccharomyces cerevisiae. Vorvis C, Markus SM, Lee WL. Yeast. 2008 Sep;25(9):651-9. 10.1002/yea.1611 PubMed 18727145