Skip to main content
Addgene

915-Plakophilin 2a-Flag
(Plasmid #32230)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32230 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pFLAG-CMV-5
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4682
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Plakophilin 2a
  • Alt name
    PKP2a
  • Species
    H. sapiens (human)
  • Entrez Gene
    PKP2 (a.k.a. ARVD9)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer CMV-Fwd
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    915-Plakophilin 2a-Flag was a gift from Kathleen Green (Addgene plasmid # 32230 ; http://n2t.net/addgene:32230 ; RRID:Addgene_32230)

  • For your References section:

    Protein binding and functional characterization of plakophilin 2. Evidence for its diverse roles in desmosomes and beta -catenin signaling. Chen X, Bonne S, Hatzfeld M, van Roy F, Green KJ. J Biol Chem. 2002 Mar 22;277(12):10512-22. Epub 2002 Jan 14. 10.1074/jbc.M108765200 PubMed 11790773
Related items:
Commonly requested with: