Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32230||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4682
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePlakophilin 2a
SpeciesH. sapiens (human)
- Promoter CMV
/ Fusion Protein
- FLAG (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer CMV-Fwd (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:915-Plakophilin 2a-Flag was a gift from Kathleen Green (Addgene plasmid # 32230 ; http://n2t.net/addgene:32230 ; RRID:Addgene_32230)
For your References section:Protein binding and functional characterization of plakophilin 2. Evidence for its diverse roles in desmosomes and beta -catenin signaling. Chen X, Bonne S, Hatzfeld M, van Roy F, Green KJ. J Biol Chem. 2002 Mar 22;277(12):10512-22. Epub 2002 Jan 14. 10.1074/jbc.M108765200 PubMed 11790773