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476-Desmocollin 2-myc
(Plasmid #32233)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32233 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 32233-D50 50 μg of DNA in Tris buffer $495
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DNA 32233-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    b-actin vector
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Desmocollin 2
  • Alt name
    DSC2
  • Species
    H. sapiens (human)
  • Entrez Gene
    DSC2 (a.k.a. ARVD11, CDHF2, DG2, DGII/III, DSC3)
  • Promoter human beta-actin
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer hBactin-F
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    476-Desmocollin 2-myc was a gift from Kathleen Green (Addgene plasmid # 32233 ; http://n2t.net/addgene:32233 ; RRID:Addgene_32233)

  • For your References section:

    Assembly of desmosomal cadherins into desmosomes is isoform dependent. Ishii K, Norvell SM, Bannon LJ, Amargo EV, Pascoe LT, Green KJ. J Invest Dermatol. 2001 Jul;117(1):26-35. 10.1046/j.0022-202x.2001.01400.x PubMed 11442746
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