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Addgene

476-Desmocollin 2-myc
(Plasmid #32233)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32233 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    b-actin vector
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Desmocollin 2
  • Alt name
    DSC2
  • Species
    H. sapiens (human)
  • Entrez Gene
    DSC2 (a.k.a. ARVD11, CDHF2, DG2, DGII/III, DSC3)
  • Promoter human beta-actin
  • Tag / Fusion Protein
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer hBactin-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    476-Desmocollin 2-myc was a gift from Kathleen Green (Addgene plasmid # 32233 ; http://n2t.net/addgene:32233 ; RRID:Addgene_32233)

  • For your References section:

    Assembly of desmosomal cadherins into desmosomes is isoform dependent. Ishii K, Norvell SM, Bannon LJ, Amargo EV, Pascoe LT, Green KJ. J Invest Dermatol. 2001 Jul;117(1):26-35. 10.1046/j.0022-202x.2001.01400.x PubMed 11442746
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