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Desmoglein2-mCherry
(Plasmid #36991)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36991 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmCherry
  • Backbone manufacturer
    K. Green lab
  • Modifications to backbone
    EGFP cDNA was replaced with mCherry in the pEGFP N1 vector (Takara Bio Inc.) using AgeI and BsrGI.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Low Kan
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dsg2
  • Alt name
    Desmoglein2
  • Species
    H. sapiens (human)
  • Entrez Gene
    DSG2 (a.k.a. CDHF5, HDGC)
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site SacII (not destroyed)
  • 5′ sequencing primer mCherry-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Desmoglein2-mCherry was a gift from Kathleen Green (Addgene plasmid # 36991 ; http://n2t.net/addgene:36991 ; RRID:Addgene_36991)
  • For your References section:

    Desmosomal cadherins utilize distinct kinesins for assembly into desmosomes. Nekrasova OE, Amargo EV, Smith WO, Chen J, Kreitzer GE, Green KJ. J Cell Biol. 2011 Dec 26;195(7):1185-203. Epub 2011 Dec 19. 10.1083/jcb.201106057 PubMed 22184201