Desmoglein2-mCherry
(Plasmid #36991)

• Depositing Lab: Kathleen Green
• Publication: Nekrasova et al J Cell Biol. 2011 Dec 26;195(7):1185-203. Epub 2011 Dec 19.

Backbone

• Vector backbone: pmCherry
• Backbone manufacturer: K. Green lab
• Modifications to backbone: EGFP cDNA was replaced with mCherry in the pEGFP N1 vector (Takara Bio Inc.) using AgeI and BsrGI.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Dsg2
• Alt name: Desmoglein2
• Species: H. sapiens (human)
• Entrez Gene: DSG2 (a.k.a. CDHF5, HDGC)
• Tag / Fusion Protein:
  • mCherry (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: SacII (not destroyed)
• 5′ sequencing primer: mCherry-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry