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1136- Desmoplakin-GFP
(Plasmid #32227)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32227 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Desmoplakin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    8616
  • Entrez Gene
    DSP (a.k.a. DCWHKTA, DP)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer CMV-Fwd
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    1136- Desmoplakin-GFP was a gift from Kathleen Green (Addgene plasmid # 32227 ; http://n2t.net/addgene:32227 ; RRID:Addgene_32227)
  • For your References section:

    Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. Godsel LM, Hsieh SN, Amargo EV, Bass AE, Pascoe-McGillicuddy LT, Huen AC, Thorne ME, Gaudry CA, Park JK, Myung K, Goldman RD, Chew TL, Green KJ. J Cell Biol. 2005 Dec 19;171(6):1045-59. 10.1083/jcb.200510038 PubMed 16365169