1127-DP NTP wild type
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32398||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1752
MutationContains only the first 584 aa
Entrez GeneDSP (a.k.a. DCWHKTA, DP)
- Promoter CMV
/ Fusion Protein
- FLAG (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer CMV-Fwd (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:1127-DP NTP wild type was a gift from Kathleen Green (Addgene plasmid # 32398 ; http://n2t.net/addgene:32398 ; RRID:Addgene_32398)
For your References section:The amino-terminal domain of desmoplakin binds to plakoglobin and clusters desmosomal cadherin-plakoglobin complexes. Kowalczyk AP, Bornslaeger EA, Borgwardt JE, Palka HL, Dhaliwal AS, Corcoran CM, Denning MF, Green KJ. J Cell Biol. 1997 Nov 3;139(3):773-84. 10.1083/jcb.139.3.773 PubMed 9348293
Map uploaded by the depositor.