Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32232||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneb-actin vector
- Backbone size w/o insert (bp) 9500
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
SpeciesH. sapiens (human)
Insert Size (bp)3362
Entrez GeneDSG2 (a.k.a. CDHF5, HDGC)
- Promoter human beta-actin
/ Fusion Protein
- Myc (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer hBactin-F (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:726-Desmoglein2-myc was a gift from Kathleen Green (Addgene plasmid # 32232 ; http://n2t.net/addgene:32232 ; RRID:Addgene_32232)
For your References section:Assembly of desmosomal cadherins into desmosomes is isoform dependent. Ishii K, Norvell SM, Bannon LJ, Amargo EV, Pascoe LT, Green KJ. J Invest Dermatol. 2001 Jul;117(1):26-35. 10.1046/j.0022-202x.2001.01400.x PubMed 11442746
Map uploaded by the depositor.