|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27281||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4095
Vector typexenopus injection
Growth in Bacteria
Gene/Insert namelow density lipoprotein receptor-related protein 6
SpeciesH. sapiens (human)
Insert Size (bp)988
Mutationcontains amino acid residues 1370–1613 (extracellular region deleted).
Entrez GeneLRP6 (a.k.a. ADCAD2, STHAG7)
/ Fusion Protein
- VSVG (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site Xbal (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Extracellular region deleted membrane tethered construct
Use Asp718 to linearize DNA for in vitro transcription by SP6
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LRP6 deltaN-pCS2-VSVG was a gift from Xi He (Addgene plasmid # 27281 ; http://n2t.net/addgene:27281 ; RRID:Addgene_27281)
For your References section:A mechanism for Wnt coreceptor activation. Tamai K, Zeng X, Liu C, Zhang X, Harada Y, Chang Z, He X. Mol Cell. 2004 Jan 16. 13(1):149-56. 10.1016/S1097-2765(03)00484-2 PubMed 14731402