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LRP6 deltaN-pCS2-VSVG
(Plasmid #27281)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27281 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    low density lipoprotein receptor-related protein 6
  • Alt name
    LRP6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    988
  • Mutation
    contains amino acid residues 1370–1613 (extracellular region deleted).
  • Entrez Gene
    LRP6 (a.k.a. ADCAD2, STHAG7)
  • Tag / Fusion Protein
    • VSVG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site Xbal (not destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

Extracellular region deleted membrane tethered construct
Use Asp718 to linearize DNA for in vitro transcription by SP6

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LRP6 deltaN-pCS2-VSVG was a gift from Xi He (Addgene plasmid # 27281 ; http://n2t.net/addgene:27281 ; RRID:Addgene_27281)
  • For your References section:

    A mechanism for Wnt coreceptor activation. Tamai K, Zeng X, Liu C, Zhang X, Harada Y, Chang Z, He X. Mol Cell. 2004 Jan 16. 13(1):149-56. 10.1016/S1097-2765(03)00484-2 PubMed 14731402