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LRP6 dC-pCS2
(Plasmid #27258)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27258 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Mammalian Expression ; xenopus injection

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    low density lipoprotein receptor-related protein 6
  • Alt name
    LRP6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4353
  • Mutation
    carboxyl intracellular region deleted Residue 1440 to internal XhoI site of gene deleted
  • Entrez Gene
    LRP6 (a.k.a. ADCAD2, STHAG7)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site Xbal (not destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Linearize with Asp718 for in vitro transcription by SP6

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LRP6 dC-pCS2 was a gift from Xi He (Addgene plasmid # 27258 ; http://n2t.net/addgene:27258 ; RRID:Addgene_27258)
  • For your References section:

    LDL-receptor-related proteins in Wnt signal transduction. Tamai K, Semenov M, Kato Y, Spokony R, Liu C, Katsuyama Y, Hess F, Saint-Jeannet JP, He X. Nature. 2000 Sep 28. 407(6803):530-5. 10.1038/35035117 PubMed 11029007