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LRP6-m5-pCS2-VSVG
(Plasmid #27283)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 27283 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    xenopus injection

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    low density lipoprotein receptor-related protein 6
  • Alt name
    LRP6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5013
  • Mutation
    Changed S/T of all five PPP(S/T)P motifs to Alanine. 2 of of the 5 sites deviate slightly from the consensus sequence (there is one PPAAP and one CPPAP)
  • Entrez Gene
    LRP6 (a.k.a. ADCAD2, STHAG7)
  • Tag / Fusion Protein
    • VSVG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site Xbal (not destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Dominant inhibitor of Wnt function, similar to LRPT-dC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LRP6-m5-pCS2-VSVG was a gift from Xi He (Addgene plasmid # 27283 ; http://n2t.net/addgene:27283 ; RRID:Addgene_27283)
  • For your References section:

    A mechanism for Wnt coreceptor activation. Tamai K, Zeng X, Liu C, Zhang X, Harada Y, Chang Z, He X. Mol Cell. 2004 Jan 16. 13(1):149-56. 10.1016/S1097-2765(03)00484-2 PubMed 14731402