159,238 results

1. pcDNA5-EGFP-NLS-P2A-mCherry-PTS1

   Plasmid

   #87803

   Purpose

   Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using P2A. NLS and PTS1 can be replaced by proteins of interest.

   Depositor

   Andrea Musacchio

   Article

   Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

   Inserts

   NLS
   PTS1
   

   Use

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter, tetracycline operator

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nrf1-Myc pcDNA3.1(+)-C-Myc

   Plasmid

   #169125

   Purpose

   expression of protein

   Depositor

   Barbara Dirac-Svejstrup

   Article

   Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

   Insert

   hNrf1 (NFE2L1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-eGFP-NDP52

   Plasmid

   #175749

   Purpose

   Lentiviral vector expressing N-terminally tagged eGFP-NDP52/CALCOCO2

   Depositor

   Wade Harper

   Article

   Heo et al Mol Cell. 2015 Oct 1;60(1):7-20. doi: 10

   Insert

   NDP52/CALCOCO2 (CALCOCO2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFR (del3) L747-E749del, A750P

   Plasmid

   #11015

   Purpose

   Retroviral construct for expressing human EGFR with L747-E749 deletion and A750P mutation in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR (del3) (EGFR Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   L747-E749 deleted, A750P

   Promoter

   Available since

   Dec. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pLVET-HA-K-RasG12V-IRES-GFP

   Plasmid

   #107140

   Purpose

   A bicistronic lentiviral mammalian expression vector for HA-KRasG12V(4B) and GFP

   Depositor

   Aki Manninen

   Article

   Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

   Insert

   HA-tagged Kras4B(G12V) and GFP (KRAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hEF-1alpha

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV4-HA

   Plasmid

   #27553

   Depositor

   Shao-Cong Sun

   Article

   Xiao et al J Biol Chem. 2000 Jul 14. 275(28):21081-

   Type

   Empty backbone

   Use

   Tags

   3x HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRBC-sfGFP wild type

   Plasmid

   #174075

   Purpose

   Plasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoter

   Depositor

   Ryan Mehl

   Article

   Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

   Insert

   Superfolder GFP
   

   Use

   Tags

   His-6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Sec61b-HA-mNG21-10

   Plasmid

   #141213

   Purpose

   Membrane Tethered Split Fluorescent Reporter for ER Retragrade

   Depositor

   Wayne Lencer

   Article

   Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

   Insert

   Sec61b-HA-mNG21-10
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCI HA NEDD4

   Plasmid

   #27002

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   NEDD4 (NEDD4 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMIH-TOMM20-Halo

    Plasmid

    #111626

    Purpose

    Fusion protein used to visualise mitochondria upon the addition of Halo specific dye, has hygromycin selection

    Depositor

    Benjamin Kile

    Article

    McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

    Insert

    TOMM20 (Tomm20 Mouse)
    

    Use

    Retroviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWZL-Neo-Myr-Flag-DEST

    Plasmid

    #15300

    Purpose

    a Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORF

    Depositor

    Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTKDP-dhfr

    Plasmid

    #71323

    Purpose

    Donor plasmid - trimethoprim resistant donor cassette

    Depositor

    Thomas Kuhlman

    Article

    Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-DIO-SYP-HRP

    Plasmid

    #117186

    Purpose

    Plasmid name in publication: pAAV-DIO-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    SYP-HRP
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS185 CMV-Cre

    Plasmid

    #11916

    Purpose

    Mammalian expression of Cre recombinase driven by the human cytomegalovirus IE promoter

    Depositor

    Brian Sauer

    Article

    Sauer et al New Biol. 1990 May . 2(5):441-9.

    Insert

    cre (cre bacteriophage P1)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMP11

    Plasmid

    #215559

    Purpose

    pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Inserts

    Cas9
    gRNA targeting pBR322 origin of replication
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23105 and tet

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pALPS env

    Plasmid

    #101325

    Purpose

    Encodes codon optimized JR-CSF env

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    JR-CSF env
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. TREM2_Halo_N_allele

    Plasmid

    #178122

    Purpose

    Donor vector for endogenous tagging of human TREM2 at the N-terminus with halotag

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Halotag (TREM2 Human)
    

    Use

    Donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCis GFPuv-SS Himar1 A7

    Plasmid

    #84686

    Purpose

    Can be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.

    Depositor

    Ulrike Munderloh

    Article

    Munderloh et al Intracellular Pathogens II: Rickettsiale

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDT-sgRNA

    Plasmid

    #138271

    Purpose

    Dual-targeting sgRNA vector that contains both a sgRNA against BFP (sgBG) and a sgRNA for a genomic target site (sgTS)

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

    Insert

    sgBG, sgTS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF2347

    Plasmid

    #144014

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HOXD8 (HOXD8 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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