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pCMMP-dnPERK-IRES-eGFP
(Plasmid #36954)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36954 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMMP-MCS-IRES-eGFP
  • Backbone manufacturer
    Addgene plasmid #36953
  • Backbone size w/o insert (bp) 7037
  • Total vector size (bp) 10074
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dnPERK
  • Alt name
    PKR-like ER kinase
  • Species
    H. sapiens (human)
  • Mutation
    deletion of aa592-1061 to create dominant negative
  • Entrez Gene
    EIF2AK3 (a.k.a. PEK, PERK, WRS)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer pLXSN_5
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Sugden lab plasmid p3236

dnPERK from Dr Ron Wek, Indiana University School of Medicine, Indianapolis.

When compared to the GenBank reference sequence, the full plasmid sequence indicates that the PERK insert contains the following polymorphisms: a deletion of amino acid L21 and Q166R and N490H mutations.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMMP-dnPERK-IRES-eGFP was a gift from Bill Sugden (Addgene plasmid # 36954 ; http://n2t.net/addgene:36954 ; RRID:Addgene_36954)
  • For your References section:

    The LMP1 oncogene of EBV activates PERK and the unfolded protein response to drive its own synthesis. Lee DY, Sugden B. Blood. 2008 Feb 15;111(4):2280-9. Epub 2007 Nov 27. 10.1182/blood-2007-07-100032 PubMed 18042799