Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31661||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepECE, Addgene plasmid 26453
- Backbone size w/o insert (bp) 2900
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namecell division cycle 27 homolog
SpeciesH. sapiens (human)
Insert Size (bp)2500
Entrez GeneCDC27 (a.k.a. ANAPC3, APC3, CDC27Hs, D0S1430E, D17S978E, H-NUC, HNUC, NUC2)
/ Fusion Protein
- M2 (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SV40pro-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pECE-M2-CDC27 wt was a gift from Anne Brunet (Addgene plasmid # 31661 ; http://n2t.net/addgene:31661 ; RRID:Addgene_31661)
For your References section:Chemical Genetic Screen for AMPKalpha2 Substrates Uncovers a Network of Proteins Involved in Mitosis. Banko MR, Allen JJ, Schaffer BE, Wilker EW, Tsou P, White JL, Villen J, Wang B, Kim SR, Sakamoto K, Gygi SP, Cantley LC, Yaffe MB, Shokat KM, Brunet A. Mol Cell. 2011 Dec 23;44(6):878-92. Epub 2011 Dec 1. 10.1016/j.molcel.2011.11.005 PubMed 22137581