|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26453||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerBill Rutter
- Backbone size (bp) 2924
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameEmpty vector
Insert Size (bp)2924
- Cloning method Restriction Enzyme
- 5′ cloning site NA (unknown if destroyed)
- 3′ cloning site NA (unknown if destroyed)
- 5′ sequencing primer SV40pro-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Plasmid kindly provided to Addgene by Dr. Anne Brunet.
There are a few single nucleotide differences and one small insertion relative to author sequence but they do not alter the MCS or affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pECE was a gift from William Rutter (Addgene plasmid # 26453 ; http://n2t.net/addgene:26453 ; RRID:Addgene_26453)
For your References section:Replacement of insulin receptor tyrosine residues 1162 and 1163 compromises insulin-stimulated kinase activity and uptake of 2-deoxyglucose. Ellis L, Clauser E, Morgan DO, Edery M, Roth RA, Rutter WJ.. Cell. 1986 Jun 6;45(5):721-32. 10.1016/0092-8674(86)90786-5 PubMed 3518947