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pECE
(Plasmid #26453)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 26453 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pECE
  • Backbone manufacturer
    Bill Rutter
  • Backbone size (bp) 2924
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Empty vector
  • Insert Size (bp)
    2924

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer SV40pro-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Plasmid kindly provided to Addgene by Dr. Anne Brunet.

There are a few single nucleotide differences and one small insertion relative to author sequence but they do not alter the MCS or affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pECE was a gift from William Rutter (Addgene plasmid # 26453 ; http://n2t.net/addgene:26453 ; RRID:Addgene_26453)
  • For your References section:

    Replacement of insulin receptor tyrosine residues 1162 and 1163 compromises insulin-stimulated kinase activity and uptake of 2-deoxyglucose. Ellis L, Clauser E, Morgan DO, Edery M, Roth RA, Rutter WJ.. Cell. 1986 Jun 6;45(5):721-32. 10.1016/0092-8674(86)90786-5 PubMed 3518947