159,238 results

1. pET21a - NR

   Plasmid

   #89625

   Purpose

   Expresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Nitrate reductase
   

   Use

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBMN FKBP(DD)-YFP

   Plasmid

   #31763

   Depositor

   Thomas Wandless

   Article

   Banaszynski et al Cell. 2006 Sep 8;126(5):995-1004.

   Insert

   FKBP(DD)-YFP-HA (FKBP1A Human)
   

   Use

   Retroviral

   Tags

   HA and YFP

   Expression

   Mutation

   F36V, L106P on FKBP12

   Promoter

   LTR

   Available since

   Oct. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. ymScarlet

   Plasmid

   #111917

   Purpose

   yeast optimized mScarlet

   Depositor

   Patrick Lajoie

   Article

   Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

   Insert

   ymScarlet
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-MH6-RspCas13d_RspCasWYL1

   Plasmid

   #108304

   Purpose

   Expresses E. coli codon optimized RspCas13d and RspCasWYL1 in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

   Inserts

   RspCas13d
   RspWYL1
   

   Use

   CRISPR

   Tags

   mH6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-mEGFP-DVL1-WT

   Plasmid

   #194586

   Purpose

   Mammalian Expression of mEGFP-DVL1 WT Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   DVL1 (DVL1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AiP11851-pAAV-hDLX-minBG-iCre-4X2C-WPRE3-BGHpA (Alias: CN1851)

   Plasmid

   #164450

   Purpose

   AAV vector for Cre expression in forebrain GABAergic interneurons. For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors.

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Article

   Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

   Has Service

   AAV PHP.eB

   Insert

   iCre
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBetaGlobin

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJS167

   Plasmid

   #48881

   Purpose

   Positive feedback plasmid from oscillator

   Depositor

   Jeff Hasty

   Article

   Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi

   Inserts

   araC gene with ssrA tag
   monomeric yeast-enhanced green fluorescent protein
   

   Use

   Synthetic Biology

   Tags

   ssrA (TSAANDENYALAA)

   Expression

   Bacterial

   Mutation

   Promoter

   Para/lac

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJS169

   Plasmid

   #48882

   Purpose

   Negative feedback plasmid from oscillator

   Depositor

   Jeff Hasty

   Article

   Stricker et al Nature. 2008 Nov 27;456(7221):516-9. doi

   Insert

   lacI with ssrA tag
   

   Use

   Synthetic Biology

   Tags

   ssrA (TSAANDENYALAA)

   Expression

   Bacterial

   Mutation

   Promoter

   Para/lac

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBMN(CMV-copGFP-Luc2-Puro)

   Plasmid

   #80389

   Purpose

   To make lentiviral vector expressing copGFP, firefly Luciferase and puromycin selection marker

   Depositor

   Magnus Essand

   Article

   Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

   Insert

   copGFP-T2A-Luc2-F2A-Puromycin
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLPC-NFLAG TRF1

    Plasmid

    #16058

    Depositor

    Titia de Lange

    Article

    Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

    Insert

    hTRF1 (TERF1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    aa2-439

    Promoter

    Available since

    Nov. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pBA900

    Plasmid

    #122237

    Purpose

    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs2 incorporated at the 3' end of the sgRNA constant region.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    sgRNA with cs2 at the 3' end of the constant region
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. mApple-PCNA-19-NLS-4

    Plasmid

    #54937

    Purpose

    Localization: Nucleus, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    PCNA-19 NLS (PCNA Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pSIN-PAmCherry-mSNCA-NE

    Plasmid

    #102364

    Purpose

    Lentiviral overexpression of PA-mCherry synuclein alpha fusion

    Depositor

    Shu Leong Ho

    Article

    Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

    Inserts

    PA-mCherry
    synuclein alpha (Snca Mouse)
    

    Use

    Lentiviral

    Tags

    NE

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. mNeonGreen-Rab7

    Plasmid

    #129603

    Purpose

    In vivo visualization of Rab7 (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    Rab7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPR-HOT-P2A-Clover-BlastR

    Plasmid

    #138568

    Purpose

    Universal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJ

    Depositor

    Hans Clevers

    Article

    Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

    Insert

    P2A-clover-BlastR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. CHOP promoter (-649/+136) pmCherry-1

    Plasmid

    #36035

    Depositor

    Quan Lu

    Article

    Oh et al J Biol Chem. 2012 Feb 17;287(8):6025-34.

    Insert

    CHOP promoter (-647 to +136) (DDIT3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)

    Plasmid

    #160611

    Purpose

    Transcriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    P35s:LbCas12a:T35s
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. PfICH pET15b

    Plasmid

    #60684

    Purpose

    Recombinant protein expression of Isocyanide Hydratase from Pseudomonas fluorescens

    Depositor

    Mark Wilson

    Article

    Lakshminarasimhan et al J Biol Chem. 2010 Sep 17;285(38):29651-6

    Insert

    inhA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

19. PS-CFP2-TBRII-N-18

    Plasmid

    #57249

    Purpose

    Localization: Focal Adhesions, Excitation: 400 / 490, Emission: 468 / 511

    Depositor

    Michael Davidson

    Insert

    TBRII (TGFBR2 Human)
    

    Use

    Tags

    PS-CFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pOpen-Eco31I

    Plasmid

    #165506

    Purpose

    Type II restriction enzyme that recognized the double-stranded sequence. GGTCTC (N) and cleaves after N-1; isoschizomer of BsaI.

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    Eco31I
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge