165,688 results

1. PB_TRE_sg83-PDGFR_EF1a_iRFP670

   Plasmid

   #186311

   Purpose

   Plasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   sg83-PDGFR
   iRFP670
   

   Tags

   His, myc

   Expression

   Mammalian

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB_TRE_sg88-PDGFR_EF1a_eBFP2

   Plasmid

   #186312

   Purpose

   Plasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   sg88-PDGFR
   eBFP2
   

   Tags

   His, myc

   Expression

   Mammalian

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB_TRE_P9-PDGFR_EF1a_mPlum

   Plasmid

   #186307

   Purpose

   Plasmid for inducible mammalian cell expression of the P9 helixCAM and constitutive expression of an identifying fluorescent protein (mPlum). Flanked with PiggyBac sites for easy line construction.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   P9-PDGFR
   mPlum
   

   Tags

   HA, myc

   Expression

   Mammalian

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB_TRE_AP10-PDGFR_EF1a_mOrange

   Plasmid

   #186308

   Purpose

   Plasmid for inducible mammalian cell expression of the AP10 helixCAM and constitutive expression of an identifying fluorescent protein (mOrange). Flanked with PiggyBac sites for easy line construction

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   AP10-PDGFR
   mOrange
   

   Tags

   HA, myc

   Expression

   Mammalian

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB_TRE_P3-PDGFR_EF1a_iRFP670

   Plasmid

   #186305

   Purpose

   Plasmid for inducible mammalian cell expression of the P3 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction.

   Depositor

   George Church

   Article

   Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

   Inserts

   P3-PDGFR
   iRFP670
   

   Tags

   HA, myc

   Expression

   Mammalian

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTriExRhoA2G

   Plasmid

   #40176

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   RhoA Biosensor
   

   Tags

   His-Myc

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET His6 MBP TEV LIC cloning vector (2M-T)

   Plasmid

   #29708

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, MBP, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe RFP1-KRT5 hygro

   Plasmid

   #58493

   Purpose

   mammalian expression of mRFP1-tagged human KRT5

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

   Insert

   KRT5 (KRT5 Human)
   

   Tags

   mRFP1

   Expression

   Mammalian

   Available Since

   Aug. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pKM446 (Flag-DAS+4))

   Plasmid

   #108321

   Purpose

   Orbit integrating plasmid for C-terminal tagging with degradation tag DAS+4, using hygromycin as a selection marker.

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Insert

   attB-Flag-DAS+4
   

   Use

   Orbit integrating plasmid

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. C1(1-29)-miniTurbo-V5_pLX304

    Plasmid

    #107176

    Purpose

    expresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vector

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Lentiviral

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-EFS-MCP-HALOnls

    Plasmid

    #121937

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    MCP-HALOnls
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP1-10-miniCMV-GFP11×11-GFP-tDeg

    Plasmid

    #185404

    Purpose

    Expresses GFP1-10 and GFP11×11-GFP-tDeg

    Depositor

    Jian-Hui Jiang

    Article

    Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

    Insert

    GFP1-10
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. PT2/C-Luc//PGK-SB13

    Plasmid

    #20207

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    Flirefly luciferase/Sb transposase
    

    Use

    Transposon

    Available Since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-SIN-BX SpyCatcher003-hCD52

    Plasmid

    #210565

    Purpose

    Expresses Spycatcher003 on the cell surface using human CD52 hinge

    Depositor

    Omer Dushek

    Article

    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

    Insert

    Spycatcher003 fused to human CD52 hinge
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSP1E3

    Plasmid

    #20064

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1E3 (APOA1 synthetic gene)
    

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1; contains repeats of he…

    Available Since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGCP123-EbpA_g1

    Plasmid

    #153517

    Purpose

    sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

    Depositor

    Kimberly Kline

    Article

    Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

    Insert

    pnisA-sgRNA(EbpA_g1)-dCas9 scaffold
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSET iGluSnFR3 v857

    Plasmid

    #175186

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria.

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    iGluSnFR3 v857
    

    Use

    Synthetic Biology

    Tags

    His tag, T7 leader, Xpress epitope

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSUPERretro-Sirt7 shRNA2

    Plasmid

    #53145

    Purpose

    shRNAi vector for Sirt7

    Depositor

    Katrin Chua

    Article

    Barber et al Nature. 2012 Jul 5;487(7405):114-8. doi:

    Insert

    Sirt7
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Promoter

    H1 RNA polymerase III

    Available Since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pSUPERretro-Sirt7 shRNA1

    Plasmid

    #53144

    Purpose

    shRNAi vector for Sirt7

    Depositor

    Katrin Chua

    Article

    Barber et al Nature. 2012 Jul 5;487(7405):114-8. doi:

    Insert

    Sirt7
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Promoter

    H1 RNA polymerase III

    Available Since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

20. CMV500 empty vector

    Plasmid

    #33348

    Depositor

    Charles Vinson

    Article

    Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

    Type

    Empty backbone

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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