161,570 results

1. pAR013 Lenti pEF-dEnAsCas12a-NLS-VP64-3XHA-T2A-BSD

   Plasmid

   #195547

   Purpose

   dCas12a effector fusion for manipulating transcription

   Depositor

   Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Lenti pEF-dEnAsCas12a-NLS-VP64-3XHA-T2A-BSD
   

   Use

   CRISPR and Lentiviral

   Tags

   3XHA

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-tTRKRAB-red

   Plasmid

   #12250

   Depositor

   Didier Trono

   Article

   Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

   Insert

   TetR-KRAB fusion and dsRED
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   dsRed added to backbone.

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSinREP5-iAchSnFR

   Plasmid

   #140643

   Purpose

   Acetylcholine sensor for sindbis virus

   Depositor

   J. Julius Zhu

   Article

   Borden et al bioRxiv 2020

   Insert

   Ach sensor V9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSMALB-ATF4.14

   Plasmid

   #155034

   Purpose

   Fluorescent reporter for ATF4 translation, Positive Control

   Depositor

   John Dick, Peter van Galen

   Article

   van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

   Inserts

   ATF4 5' UTR (Atf4 Mouse)
   ATF4-eGFP fusion ORF
   TagBFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   uORF1 and uORF2 start codons mutated

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBabe_Hyg_cOVA_T2A_mStrawberry

   Plasmid

   #161737

   Purpose

   Expression of cOVA and mStrawberry

   Depositor

   Alec Kimmelman

   Article

   Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

   Insert

   cytoplasmic ovalbumin
   

   Use

   Retroviral

   Tags

   mStrawberry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. OMM-V5-LOV-Turbo

   Plasmid

   #199665

   Purpose

   expresses LOV-Turbo on the mammalian outer mitochondrial membrane, lentiviral vector

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Use

   Lentiviral

   Tags

   AKAP1 transmembrane domain and V5

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. FKBP-mEGFP-YAP-MAML2

   Plasmid

   #217612

   Purpose

   part A of YAP-MAML2 SPARK-OFF

   Depositor

   Xiaokun Shu

   Article

   Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

   Insert

   FKBP-mEGFP-YAP-MAML2 (YAP1, MAML2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Pet28a_Human_H4

   Plasmid

   #42633

   Depositor

   Joe Landry

   Insert

   Human H4 (H4C1 Human)
   

   Use

   Tags

   Untagged

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 25, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pKL2351

   Plasmid

   #199721

   Purpose

   A pVS1-based T-DNA binary vector and destination vector for Gateway cloning.

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   NPTII
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. AICSDP-22:CETN2-mTagRFP-T

    Plasmid

    #101781

    Purpose

    Homology arms and mTagRFPT-linker sequence for N-terminus tagging of human CETN2

    Depositor

    Allen Institute for Cell Science

    Insert

    CETN2 Homology Arms with mTagRFPT-linker (CETN2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mTagRFPT-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Oct. 31, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pQE 80L Kan T7WT

    Plasmid

    #102790

    Purpose

    Expresses T7 RNA polymerase

    Depositor

    Andrew Ellington

    Article

    Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

    Insert

    T7 RNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 3, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pcDNA4/TO-HRASG12V

    Plasmid

    #216509

    Purpose

    Expresses HRAS with a G12V point mutation in human cells under a tetracycline/doxycycline-inducible promoter

    Depositor

    John Diffley

    Article

    Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

    Insert

    HRAS (HRAS Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG mito-GCaMP5G

    Plasmid

    #105009

    Purpose

    mitochondrial calcium sensor

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    GCaMP5G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET22b_PstS_1

    Plasmid

    #78198

    Purpose

    Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

    Depositor

    Martin Webb

    Article

    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

    Insert

    pstS (A197C)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A197C (please see depositors comment below)

    Promoter

    T7

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHIV-H2B-eGFP

    Plasmid

    #91776

    Purpose

    Expresses H2B protein tagged with eGFP

    Depositor

    Maria Pia Cosma

    Article

    Sottile et al Sci Rep. 2016 Nov 9;6:36863. doi: 10.103

    Insert

    Human Histone H2B / eGFP (H2BC11 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF0831

    Plasmid

    #142208

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZBTB33 (ZBTB33 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-Tag2B EDD

    Plasmid

    #37188

    Depositor

    Charles Watts, Darren Saunders

    Article

    Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

    Insert

    EDD (UBR5 Human)
    

    Use

    Tags

    6x His Tag and FLAG

    Expression

    Mammalian

    Mutation

    Please see Depositor Comments

    Promoter

    CMV

    Available since

    Sept. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBAD-LSSmCherry1

    Plasmid

    #89986

    Purpose

    Long stokes-shift variant of red fluorescent protein mCherry

    Depositor

    Robert Campbell

    Article

    Shen et al PLoS One. 2017 Feb 27;12(2):e0171257. do

    Insert

    LSSmCherry1
    

    Use

    Tags

    6xHis-T7-Xpress

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUK21

    Plasmid

    #49788

    Purpose

    E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)

    Depositor

    Joachim Messing

    Article

    Vieira et al Gene. 1991 Apr;100:189-94.

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    lacZ

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-PS11-scFvFc-CD28-gp41 (706-713)

    Plasmid

    #60606

    Purpose

    Mammalian Display vector

    Depositor

    Wayne Marasco

    Article

    Taube et al PLoS One. 2008 Sep 11;3(9):e3181. doi: 1

    Insert

    PS11-scFvFc-CD28-gp41 (706-713) (CD28 Human)
    

    Use

    Tags

    C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2014

    Availability

    Academic Institutions and Nonprofits only