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The Dynamics of mRNA Turnover Revealed by Single-Molecule Imaging in Single Cells.
Horvathova I, Voigt F, Kotrys AV, Zhan Y, Artus-Revel CG, Eglinger J, Stadler MB, Giorgetti L, Chao JA
Mol Cell. 2017 Nov 2;68(3):615-625.e9. doi: 10.1016/j.molcel.2017.09.030. Epub 2017 Oct 19.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
104094pSF4 TetCMV intron Renilla STOP 12xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyAExpresses TREAT reporter in mammalian cells
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104095pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyAExpresses TREAT-siRNA reporter in mammalian cells
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104096pSF4 TetCMV intron Renilla STOP 12xPP7 TNFα-ARE xrRNA12 24xMS2 SV40 CTE polyAExpresses TREAT-ARE reporter in mammalian cells
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104097pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA 24xMS2 SV40 CTE polyAExpresses TREAT-PK co-localization control in mammalian cells
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104098phage UbiC NLS HA stdMCP HaloLentiviral vector expressing synonymous tandem MS2 coat protein fused to Halo. Used to label reporter mRNAs.
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104099phage UbiC NLS HA stdPCP stdGFPLentiviral vector expressing synonymous tandem PP7 coat protein fused to synonymous tandem GFP. Used to label reporter mRNAs.
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104140pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyAExpresses TREAT-5'TOP reporter in mammalian cells
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Antibodies from Article