pBABE_hygro_mRFP1_NRF2
(Plasmid #136579)

• Purpose: mammalian expression of mRFP1-tagged human NRF2
• Depositing Lab: Kevin Janes
• Publication: Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13/627/eaba4200. doi: 10.1126/scisignal.aba4200.

Backbone

• Vector backbone: pBABE-hygro
• Backbone manufacturer: Addgene #1765
• Backbone size w/o insert (bp): 5219
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NRF2
• Species: H. sapiens (human)
• Insert Size (bp): 2530
• GenBank ID: NM_006164
• Entrez Gene: NFE2L2 (a.k.a. HEBP1, IMDDHH, NRF2, Nrf-2)
• Tag / Fusion Protein:
  • N-mRFP1-FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: pBABE 5'

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry