PurposeBlue-light dependent heterodimerization system
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||162247||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5354
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Insert Size (bp)1281
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please visit https://doi.org/10.1101/2020.08.28.272807 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:eMagAF-EGFP-PM was a gift from Pietro De Camilli (Addgene plasmid # 162247 ; http://n2t.net/addgene:162247 ; RRID:Addgene_162247)
For your References section:Optimized Vivid-derived Magnets photodimerizers for subcellular optogenetics in mammalian cells. Benedetti L, Marvin JS, Falahati H, Guillen-Samander A, Looger LL, De Camilli P. Elife. 2020 Nov 11;9. pii: 63230. doi: 10.7554/eLife.63230. 10.7554/eLife.63230 PubMed 33174843